图５里有两个predicted. 我不是很清楚阎润涛说的是哪个， 就两个都谈谈我的理解。
一： Shown here are the predicted conformations—outward-open, ligand-bound and occluded, inward-open, and ligand-free and occluded—required for a complete transport cycle according to the alternating access model.
粗体的部分我不是很清楚是应该理解为：
（１）Shown here are the conformations predicted by the scientific community. 还是
（2） Shown here are the conformation predicted through our experiments.
如果是（1）那么没问题。如果是（2）需要先解释第二个predict.
二。 The ligand-bound, occluded conformation and the ligand-free, occluded one are predicted from two XylE structures in the outward-facing, partly occluded and ligand-bound state
Accordingly, this model may be thought of as describing a MOBILE BARRIER (22); ...If. condition ... (then) one might also view this transporter as a "GATED PORE"
“when the transporter is in its outward-facing conformation, external substrates (or probes) would contact domains C and B but not domain A. Conversely, when in the inward-facing conformation, substrates (or probes) could interact with domains A and B but not with domain C. Accordingly, this model may be thought of as describing a MOBILE BARRIER (22); substrate transport arises as the formation and collapse of barriers (domains A and C) forces the translocation pathway to communicate with the inner and outer aqueous phases in alternating sequence. If the core domain B functions solely as a passive conduit for substrate movement, one might also view this transporter as a "GATED PORE" but before adopting this terminology it may be best to await a better experimental definition of this central region.”
注22. Mitchell, P. (1990) Res. Microbiol. 141, 286-289
“when the transporter is in its outward-facing conformation, external substrates (or probes) would contact domains C and B but not domain A. Conversely, when in the inward-facing conformation, substrates (or probes) could interact with domains A and B but not with domain C. Accordingly, this model may be thought of as describing a MOBILE BARRIER (22); substrate transport arises as the formation and collapse of barriers (domains A and C) forces the translocation pathway to communicate with the inner and outer aqueous phases in alternating sequence. If the core domain B functions solely as a passive conduit for substrate movement, one might also view this transporter as a "GATED PORE" but before adopting this terminology it may be best to await a better experimental definition of this central region.”
注22. Mitchell, P. (1990) Res. Microbiol. 141, 286-289
就因为在7月15日的帖子“转运蛋白模型一日游 （五）总结”的最初版本中，我和你讨论，你说“目前看过的文献来看， Yan_Maloney模型只出现在阎的博客里，没有出现在科学杂志里，就是说在科学界不存在”。我只好问你“‘The best characterized members of the family are UhpT and GlpT, both of E. coli, for which detailed topological models have been presented (29, 90, 91).’ 这是被引用了1281次的综述“Major facilitator superfamily（1998）”文章里的原话。其中文献90， 91分别是大阎1993和1995的文章。队友阁下，在这里"models"是啥意思？”。我再说“这就是你‘看了这么多文献，没有一篇文献用模型一词来提阎的两篇论文’。反证”，到这时你才无奈地承认你错了，有大阎的模型。
