1.Duran-Prado M, et al. J Clin Endocrinol Metab (2009) 94:2634–2643
Identification and characterization of two novel truncated but functional isoforms of the somatostatin receptor subtype 5 differentially present in pituitary tumors.
2.Cordoba-Chacon J, et al. Cell. Mol. Life Sci. (2010) 67:1147–1163
Identification and characterization of new functional truncated variants of somatostatin receptor subtype 5 in rodents
3.Perron A, et al. J Biol Chem (2005) 280 (11): 10219-27
Identification and Functional Characterization of a 5-Transmembrane Domain Variant Isoform of the NTS2 Neurotensin Receptor in Rat Central Nervous System
4.Roland J, et al. Blood (2003) 101 (2): 399-406
Identification and characterization of new functional truncated variants of somatostatin receptor subtype 5 in rodents
Cellular and Molecular Life Sciences volume 67, pages1147–1163(2010
5.Hamatake M, et al. Cancer Sci. (2009) 100 (1): 95-102
Ligand-independent higher-order multimerization of CXCR4, a G-protein-coupled chemokine receptor involved in targeted metastasis.
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回复 'Stegy223' 的评论 : Identification and Functional Characterization of a 5-Transmembrane Domain Variant Isoform of the NTS2 Neurotensin Receptor in Rat Central Nervous System
Article (PDF Available)?in?Journal of Biological Chemistry 280(11):10219-27?·?March 2005?with?18 Reads?
The present study demonstrated that alternative splicing of the rat nts2 receptor gene generates a 5-transmembrane domain variant isoform (vNTS2) that is co-expressed with the full-length NTS2 receptor throughout the brain and spinal cord, as evidenced by reverse transcription-PCR. The vNTS2 polypeptide is 281 amino acids in length, which is 135 amino acids shorter than the full-length isoform. Immunohistochemical and radioligand binding studies revealed that the HA-tagged recombinant vNTS2 receptor is poorly targeted to plasma membranes in transfected COS-7 cells. Binding studies also showed that the truncated receptor displayed a 5000-fold lower affinity for neurotensin (NT) than its full-length counterpart (IC(50) of 10 mum and 2 nm, respectively). Yet NT binding induced efficient internalization of receptor-ligand complexes in vNTS2-transfected cells. Furthermore, it produced a rapid (1 h) and is also produced by the NTS2 agonist levocabastine. Western blotting experiments suggested that vNTS2 is not expressed in monomeric form in the rat central nervous system. However, it does appear to form a variety of multimeric complexes, including homodimers and heterodimers, with the full-length NTS2. Indeed, co-immunoprecipitation studies in dually transfected cells demonstrated that the two receptor isoforms can form stable associations. Taken together, the present results indicated that the rat vNTS2 is a functional receptor that may play a role in NT signaling in mammalian central nervous system.
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回复 'Stegy223' 的评论 : 有啊,五个以下TM被证明有生物活性,有人还证明了裴刚的结论。我没看这些文章:1.Duran-Prado M, et al. J Clin Endocrinol Metab (2009) 94:2634–2643
2.Cordoba-Chacon J, et al. Cell. Mol. Life Sci. (2010) 67:1147–1163
3.Perron A, et al. J Biol Chem (2005) 280 (11): 10219-27
4.Roland J, et al. Blood (2003) 101 (2): 399-406
5.Hamatake M, et al. Cancer Sci. (2009) 100 (1): 95-102