还有,我很好奇《自然》杂志的回信有没有我这封《自然杂志的回复》客气。大阎,大家都等了这么久了,给大家看看吧!至少要给你那些粉看看呀,你看他们对你多好:"A YRT's fan from WXC, I hereby give my unconditional, continuingsupport to YRT. This is also to prove that here is an open platform where everyone can post comments at whatever time and in whatever way one pleases.",人家可是给你无条件的支持的!
Moving my comment to Yan to your space so I may add more later (although it has been a waste of my time). thanks
回复 '山丘-' 的评论 : AAM模型理论研究进展在那里(1966-2013结果),颜宁二零一四文中图五就是按理论画的。为了提出2014working model, 就需要更多于2013内容。向内开口构象已经被拍到,无争论,向外开构象,待定,但是小颜在2014文中讨论部分从结构方面作了很好的说明(由于TMD及ICH作用,没有底物时的最可能构象是向外开,底物结合及分离则促进二个构象转换。这种讨论不需要其他数据)。至于AAM机理要求两个occluded 结构,则需要借助XyLE, 那也是现成的(文献28,29),也是小颜的,且已经有结晶及生化数据。因此她在2014文中说了our... biochemical data。没什么看不懂的吧。既然2014拿到GluT1的结构,此时提出working model,顺理成章。与老阎老板无关,66-92,92-2013之间AAM模型早在那,内含逐年增加。与老阎九三年模式无关。至于老阎九三文章,那个示意图根本没谈机理,只是标出了三个区域而异。文章写道:although we can identify a residual within the translocation pathway of UhPT, we can not yet comment on how this pathway may operate”. 对于这篇文章的贡献与局限性,可以作很多评论。
Yan's paper in 1993 is about identifying a residue C265 and 3 domain. The famous graph he used is not about mechanism, but about 3 domain with C265 in the core domain. As for pathway mechanism, they did not know and suggested mobile carrier (c265 as elevator) and mobile barrier (gated pore) theories. They are not related to outward open/inward open conformation Yan Nieng talked about. So older Yan started with a lie. Shocking?